槲皮素与5-氨基水杨酸协同治疗感染后肠易激综合征大鼠的药效机制

张玉春, 左晓, 邵云云, 饶志, 武新安, 秦红岩

中国医院药学杂志 ›› 2016, Vol. 36 ›› Issue (23) : 2054-2058.

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中国医院药学杂志 ›› 2016, Vol. 36 ›› Issue (23) : 2054-2058. DOI: 10.13286/j.cnki.chinhosppharmacyj.2016.23.04
研究论文

槲皮素与5-氨基水杨酸协同治疗感染后肠易激综合征大鼠的药效机制

  • 张玉春1, 左晓1,2, 邵云云1,2, 饶志1, 武新安1, 秦红岩1
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Synergistic effects and underlying mechanism of quercetin and 5-aminosalicylic acid on post-infactious irritable bowel syndrome in rats

  • ZHANG Yu-chun1, ZUO Xiao1,2, SHAO Yun-yun1,2, RAO Zhi1, WU Xin-an1, QIN Hong-yan1
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摘要

目的:研究槲皮素与5-氨基水杨酸(5-ASA)对感染后肠易激综合征(PI-IBS)的治疗作用及其机制。方法:PI-IBS大鼠模型分别灌胃给予5-ASA、槲皮素或5-ASA联合槲皮素,连续给药2周。腹壁收缩实验测定内脏痛阈值,水应激法评价结肠运动;收集血液和结肠,液质联用法测定5-ASA及其代谢物N-乙酰-5-ASA含量。结果:与正常组相比,PI-IBS组内脏痛阈值显著降低,结肠排便增多(P<0.05)。与模型组相比,5-ASA联合槲皮素可显著提高PI-IBS大鼠内脏痛阈值并明显减少结肠排便数量,其作用优于各单独用药组。槲皮素可明显增加PI-IBS大鼠结肠5-ASA含量并显著降低N-乙酰-5-ASA含量(P<0.05)。结论:槲皮素联合5-ASA对PI-IBS大鼠具有协同治疗作用,其机制可能与槲皮素抑制5-ASA代谢酶,提高结肠5-ASA浓度从而增强其治疗效果有关。

Abstract

OBJECTIVE To investigate therapeutic effects and mechanism of quercetin and 5-aminosalicylic acid (5-ASA) on post-infectious irritable bowel syndrome (PI-IBS) in rats.METHODS PI-IBS rats were orally administered 5-ASA, quercetin or 5-ASA plus quercetin, respectively. Drugs were given to rats for 2 weeks consecutively. After treatment, visceral pain threshold pressure was measured by abdominal withdrawal reflex test, while colonic transit was evaluated by water avoidance stress test in PI-IBS rats. Then blood samples and colon tissues were collected for determination of concentrations of 5-ASA and its metabolite N-acetyl-5-ASA (N-AC-5-ASA) by LC-MS/MS method.RESULTS Compared to normal rats, PI-IBS rats presented significantly reduced visceral pain threshold pressure and increased fecal pellet output (P<0.05). Compared to PI-IBS model rats, pain threshold pressure was markedly increased, while fecal pellet output was significantly reduced in rats treated with 5-ASA in combination with quercetin, and this therapeutic effect was superior to that of rats treated with 5-ASA or quercetin only. Moreover, quercetin treatment significantly increased content of 5-ASA but markedly reduced content of N-AC-5-ASA in colon tissues of PI-IBS rats (P<0.05).CONCLUSION Synergistic therapeutic effects are observed in PI-IBS rats treated with 5-ASA in combination with quercetin, and underlying mechanism may be correlated with inhibitory effects of quercetin on 5-ASA metabolic enzyme, which increases colonic concentration of 5-ASA, and thus improves therapeutic effects of 5-ASA in PI-IBS rats.

关键词

感染后肠易激综合征 / 槲皮素 / 5-氨基水杨酸 / 内脏痛敏

Key words

post-infectious irritable bowel syndrome / quercetin / 5-aminosalicylic acid / visceral hyperalgesia

引用本文

导出引用
张玉春, 左晓, 邵云云, 饶志, 武新安, 秦红岩. 槲皮素与5-氨基水杨酸协同治疗感染后肠易激综合征大鼠的药效机制[J]. 中国医院药学杂志, 2016, 36(23): 2054-2058 https://doi.org/10.13286/j.cnki.chinhosppharmacyj.2016.23.04
ZHANG Yu-chun, ZUO Xiao, SHAO Yun-yun, RAO Zhi, WU Xin-an, QIN Hong-yan. Synergistic effects and underlying mechanism of quercetin and 5-aminosalicylic acid on post-infactious irritable bowel syndrome in rats[J]. Chinese Journal of Hospital Pharmacy, 2016, 36(23): 2054-2058 https://doi.org/10.13286/j.cnki.chinhosppharmacyj.2016.23.04
中图分类号: R285.5   

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基金

国家自然科学青年基金项目(81400596),甘肃省卫生行业科研计划管理基金项目(GWGL2013-25)

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